Posted by on April 22, 19102 at 18:58:30:

Sir,
Subject: “Application for the position of Research Scientist/PDF/RA” - reg.

I successfully completed my Ph.D., thesis in plant tissue culture entitled " In vitro culture studies in horsegram [Macrotyloma uniflorum (Lam.) Verdc.]. At present I am involved in Genetic transformation of legume crops for insect and viral resistance via Agrobacterium and Particle Bombardment. I am looking for a suitable position. As I understand that there may be some vacancy for the posts of Post Doctoral/ Research Associate / Scientists position in your esteemed institute, I shall eager to apply for the ongoing year 2001-2002. I have the relevant experience and expertise in pursuing the job. I have so far published (National and International) papers and have attended conferences. Since I have a burning urge to take up research as my career. I am sure that I shall do my best on taking up the offer. I am herewith enclosing my brief resume and abstract of current research activities, for your kind perusal. Looking forward to hear from you.
Thanking you,
Yours sincerely,

S.VARISAI MOHAMED
Room No. 308
School of Life Sciences
Jawaharlal Nehru University
New Delhi – 110067, India.
Email: varis72@rediffmail.com

Bio-data

Name: Dr. S. Varisai Mohamed

Address: Room No. 308, School of Life Sciences,
Jawaharlal Nehru University, New Delhi – 11 0067,
INDIA.
E-mail: varsaimohamed@yahoo.com, varis72@rediffmail.com
Fax: (0091-6187338)
Phone: (0091– 6107676 Ext. No. 2523

Educational qualifications:

Bharathidasan University, India Ph.D. 2000
Botany (Plant Biotechnology)

Bharathidasan University, India M.Sc., 1994
Botany
Research experience:
2001 August-Present) - Research Associate (Department of Biotechnology, Govt. of
India), (“ Mass scale propagation of Litchi ”).
2000-2001 (May to August) - Research Associate (Council of Scientific Industrial
Research, Govt. of India.)
(“Pyramiding of insect resistance gene in pigeonpea
(Cajauns cajan L. Mill.sp) plants by particle
bombardment of meristems”)

1994-2000 Doctoral studies at Bharathidasan University,
Tiruchirappalli-620 024, TN, INDIA
(In vitro culture studies in horsegram (Macrotyloma
uniflorum (Lam.) Verdc.)

Awards & Honors:
1997-1998 Senior Research Fellow - CSIR
2000-2001 Research Associate - CSIR
2001- (Present) Research Associate –DBT, Ministry of Science & Technology,
Department of Biotechnology, New Delhi – India.
Teaching experience:
Worked as a Lecturer in Biotechnology for two months in Muthayammal College of Arts & Science, Rasipuram, Namakkal, Tamil Nadu, India.

Technical expertise:
· In vitro tissue culture techniques
· Cell suspension culture and somatic embryogenesis major grain legumes
· Agrobacterium-mediated gene transfer
· Particle bombardment
· PCR
· Southern Blotting

Research Interest:
* Molecular Basis of Agrobacterium mediated gene transformation in higher plants
* Genetic engineering of higher Plants for diseases resistance

Reference:

Dr. N.Jayabalan,
Reader in plant science
Bharathidasan University,
Tiruchirappalli,
Tamilnadu, India.
E-mail: balan@bdu.ernet.in

Prof. Neera Bhalla Sarin
Room No. 305,
School of Life Sciences,
Jawaharlal Nehru University,
New Delhi – 67, India.
Email: ceracon@vsnl.com : neera_51@hotmail.com

Dr. A. Ganapathi,
Reader in Biotechnology,
School of Life Sciences,
Bharathidasan University,
Thiruchirappalli – 622024, T.N. India.
Email: ganap@bdu.ernet.in

Dr. M.M. Mustaffa,
Principal Scientist,
National Research Centre on Banana (ICAR),
44, Ramalinga Nagar South Extn., Vayalur Road,
Tiruchirappalli-620017, T.N. India.
Fax: 0431-770564
Phone: 0431-771039
Email: nrcbaris@tr.dot.net.in

List of significant publications during last five years (with details)

S. Varisai Mohamed and N. Jayabalan (1996). A protocol for Horsegram [Macrotyloma uniflorum(Lam.) Verdc.] callus induction. Israel Journal of Plant Sciences.44: 143-145.

S. Varisai Mohamed, M. Jawahar and N. Jayabalan (1998). Effect of Ads, BAP and IBA on plant regeneration from Macrotyloma uniflorum (Lam.) Verdc. Phytomorphology 48 (1): 61-65.

S. Varisai Mohamed, M. Jawahar and N. Jayabalan (1998). Establishment of efficient method for plant regeneration from cotyledonary node explants of Macrotyloma uniflorum (Lam.) Verdc. Physiology and Molecular Biology of Plants 4: 139-142.

M. Jawahar, S. Varisai Mohamed and N. Jayabalan (1997). A simple protocol for efficient plantlet regeneration from tomato [Lycopersicon esculentum. Mill] hypocotyl derived callus. Plant Tissue Culture 7 (1): 35-39.

M. Jawahar, S. Varisai Mohamed and N. Jayabalan (1998). In vitro induction of plantlets regeneration from cotyledonary callus of Tomato (Lycopersicon esculentum. Mill). Advances in Plant Science11 (1): 199-204.

M. Jawahar, S. Varisai Mohamed and N. Jayabalan (1997). In vitro callus culture and plant regeneration from different explants of Lycopersicon esculentum. Mill. Journal of Phytological Research 10 (1-2): 75 - 78.

S. Varisai Mohamed, M. Jawahar and N.Jayabalan (1999). Plantlet regeneration from cotyledon derived calli of Horsegram [Macrotyloma uniflorum (Lam.) Verdc.] Plant Tissue Culture and Biotechnology Emerging Trends, Editor P.B. Kavi Kishor, University press. Pp 193 - 196.

S. Varisai Mohamed, M .Jawahar, M. Thiruvengadam, M. Jayakumar and N. Jayabalan
(1999) Effect of cytokinins on proliferation of multiple shoots in Horsegram [Macrotyloma uniflorum (Lam.) Verdc.]. Journal of Plant Biotechnology 1(2): 79-83.

Communicated:
S. Varisai Mohamed, M. Jawahar, and N. Jayabalan (1998). Studies on Induced
mutation frequency in Macrotyloma uniflorum Lam. Verdc. The Indian Journal of Genetics and Plant Breeding. Manuscript no. 97/68/5184/98 (revised)

S. Varisai Mohamed, V. Ramesh Anbazhagan, N. Dolendro Singh, L. Sahoo, Pawan K. Jaiwal and N. Jayabalan (2001). Plant regeneration via somatic embryogenesis through cell-suspension cultures of horsegram (Macrotyloma uniflorum (Lam.) Verdc.)
Plant Cell, Tissue and Organ Culture (Accepted for publication)

To be communicated:

S. Varisai Mohamed et al., Shoot regeneration from the callus of mature cotyledon segments in horsegram (Macrotyloma uniflorum (Lam.) Verdc.) Plant Growth Regulations.

S. Varisai Mohamed et al., In vitro plant regeneration from epicotyl derived callus of horsegram (Macrotyloma uniflorum (Lam.) Verdc.) Plant Cell tissue and organ culture

S. Varisai Mohamed and N. Jayabalan (2002) High frequency of somatic embryogenesis from cell suspension cultures using cotyledon callus of horsegram. In vitro cell and Dev. Biol.
S. Varisai Mohamed et al., (2002) Factors affecting and controlling somatic embryogenesis in cell suspension cultures of horsegram. Biologia Plantarum.

S. Varisai Mohamed, Lingaraj Sahoo, M. Thiruvengadam and V. Ramesh Anbazhagan (2002). Regeneration of plants via somatic embryogenesis using cell suspension cultures of Vigna radiata using Leaf and cotyledon explants. Plant Tissue and Organ Culture (or) Plant Cell reports.

S. Varisai Mohamed and Neera Bhalla Sarin (2002) Somatic embryogenesis in cell suspension cultures of Vigna mungo using leaf derived embryogenic callus
Plant Cell Reports.

Presentations / Conferences:
S. Varisai Mohamed and N. Jayabalan (1995). "In vitro studies in Horsegram (M.uniflorum (Lam.) Verdc.)" Seminar on recent trends in Biotechnological Research, Bharathidasan University, Tiruchirappalli.

S. Varisai Mohamed and N. Jayabalan (1995). "Induced callus variability in Horsegram (M.uniflorum (Lam.) Verdc.)" National symposium on new directions in plant biodiversity research, Bharathidasan University, Tiruchirappalli.

S. Varisai Mohamed and N. Jayabalan (1995). "Callus induction and multiple shoot formation in Horsegram (Macrotyloma uniflorum (Lam.) Verdc. National Symposium on recent trends in plant science. Osmania University, Hyderabad.

S. Varisai Mohamed and N. Jayabalan (1995). Effect of Indole - 3 - Acetic Acid: on some biochemical changes in early stages of Macrotyloma uniflorum. National Symposium on role of Crop Physiology in Improving Agricultural Productivity. TNAU, Coimbatore.

S. Varisai Mohamed and N. Jayabalan (1996). Adenine Sulphate promote shoot proliferation in Horsegram. (Macrotyloma uniflorum (Lam.) Verdc.). National Symposium on current trends in plant biochemistry and Biotechnology. CCS. Haryana Agri University Hisar.

S. Varisai Mohamed and N. Jayabalan (1996). Plantlet regeneration from cotyledon derived calli of Horsegram (Macrtyloma uniflorum). National Symposium on "Emerging trends on plant tissue culture and molecular Biology. Osmania University, Hyderabad.

S. Varisai Mohamed and N. Jayabalan (1996). Induction of multiple shoots in Horsegram (Mactrotyloma uniflorum) 84th Session of Science Congress in Delhi.

S. Varisai Mohamed and N. Jayabalan (1997). Somatic embryogenesis in Horsegram (Macrotyloma uniflorum (Lam.) Verdc.). National Seminar, Biological Diversity and Human Welfare. Govt. P.G. College, Rishikesh.

S. Varisai Mohamed and N. Jayabalan (1998). Studies on induced chlorophyll mutation frequency in Horsegram. "Induced mutations and molecular techniques in improving crop productivity and quality". M.S. Swaminathan Research Foundation, Chennai, India.

S. Varisai Mohamed and N. Jayabalan (1999). Plant regeneration from epicotyl explants of Horsegram (Macrotyloma uniflorum) National Conference on Plant Biotechnology : Towards Strategic Agriculture and Drug Development , March 15 - 17, Central Institute of Medicinal and Aromatic Plants, Lucknow, India.

S. Varisai Mohamed and N. Jayabalan (1999). Ontogeny of somatic embryos in cell suspension culture of Horsegram (Macrotyloma uniflorum (Lam.) Verdc.). International Conference on Life Science in the Next Millennium. School of Life Sciences University of Hyderabad. Pp-18:61-62.
S. Varisai Mohamed, Lingaraj Sahoo and N. Jayabalan (2001). Plant regeneration via somatic embryogenesis through cell-suspension cultures of horsegram (Macrotyloma uniflorum (Lam.) Verdc.). National Symp. On Plant Biotech. & Mol. Biol. 24th Annual Meeting of PTCA (India), University of Delhi South Campus, NewDelhi, Oct. 12-14.

Summary of Current Research Activity:

The major grain legumes of Indian subcontinent chickpea, pigeonpea, blackgram, green gram and horsegram contribute significant amount of vegetable protein to the diet of the developing countries of this region. Production of these legumes has been drastically reduced due to the severe diseases and abiotic stresses these regions experience. Breeding programs have been restricted due to narrow existing germplasm and sexual incompatibility of the cultigens with the wild varieties, the reservoir of desirable genes. Genetic engineering of these grain legumes, to elevate resistance to various diseases by incorporating desirable genes from alien sources, is being focused. However lack of efficient in vitro regeneration systems and absence of suitable transformation protocol has so far delayed the application of these cutting edge tools. I have already developed suitable regeneration systems for most of the above grain legumes particularly, pigeonpea, horsegram and blackgram besides green gram via direct organogenesis, callus mediated regeneration and somatic embryogenesis through cell suspension culture. The developed regeneration systems have been assessed for their compatibility to the routine transformation protocol. Currently I am involved in developing transgenics in pigeonpea and blackgram by incorporating insecticidal Bt and VMYMV coat protein gene respectively.