Posted by on March 05, 19102 at 05:45:52:

RÉSUMÉ

Javed Hussain Niazi. K.M
Research Fellow
Department of Biochemistry
Gulbarga University,
Gulbarga- 585 106, INDIA
Phone Off. : 91 08472 48819
Res. : 91 083 9821320
E-mail: javedjabbu@rediffmail.com

EDUCATION

B.Sc., (Chemistry, Botany, Zoology) 1994, Gulbarga University, Gulbarga, INDIA.

M.Sc., (Biochemistry), 1996, Department of Biochemistry, Gulbarga University, Gulbarga, INDIA.

Ph.D Biochemistry, 2002. Thesis submitted to Gulbarga University, Gulbarga.

*Ph.D. dissertation entitled, “Microbial Degradation of Aromatic Compounds”

EXPERTISE

Biochemical studies
ˇ Enzyme assays (on Gel - esterase, alkaline phosphatase, acid phosphatase, dehydrogenases, mono and di-oxygenases)
ˇ Biochemical analysis of most of the biomolecules- carbohydrates, DNA, RNA, lipids.

Protein Chemistry Techniques

ˇ Isolation and Characterization of plant & bacterial proteins
ˇ Familiar with most of the protein purification techniques.
ˇ Chromatography techniques-
ˇ Ion-exchange chromatography
ˇ Molecular sieve chromatography
ˇ Gel elution of proteins
ˇ Electrophoretic techniques – SDS-PAGE, Native PAGE

Other techniques
ˇ Thin layer chromatography
ˇ Paper chromatography- specially for amino acid analysis
ˇ Generation and partial purification of antibodies of chicken (IgY) and some basic molecular biology techniques.

Microbiological techniques
ˇ Isolation and maintenance of microorganisms- bacteria, bacteriophage and fungi
ˇ Microscopic studies using – light microscope, phase contrast microscope

Organic Synthesis
ˇ Synthesis of rare organic chemicals; synthesized the metabolic intermediates required as standards while elucidating the metabolic pathways during the metabolism of xenobiotics by bacteria viz., Mono-methylphthalate, Mono-ethylphthalate and other rare organic compounds.

Instruments (can handle independently)

ˇ Electrophoretic set up-protein and DNA/RNA
ˇ UV-Vis spectrophotometer
ˇ High Speed refrigerated centrifuge
ˇ Lyophilizer
ˇ Column chromatography set up
ˇ Gel documentation system
ˇ PCR
ˇ Rotary Evaporator

Highlights of Research work

The highlights of my doctoral research – Microbial Degradation of Aromatic Compounds (Bacterial Degradation of Phthalates and their esters)

ˇ Isolation, characterization, and maintenance of microorganisms, which are capable of degrading phthalates and their esters and other xenobiotics. We have few microorganisms isolated from contaminated sites near industries that are capable of utilizing phthalate esters, like dimethyl, di-n-butyl, diethylhexyl phthalates, phenol, benzene and its derivatives, naphthalene, anthracene, phenanthrene, and other toxic chemicals.

ˇ We have demonstrated that the bacterium belonging to Bacillus sp. is capable of utilizing phthalate esters (PE’s), which is used as plasticizer in manufacture of plastics, insect repellents, cosmetics, synthetic fibers, pesticide carriers etc. This was done by elucidation of metabolic pathways by isolating the metabolic intermediates of phthalate esters from the spent medium of this bacterium. The metabolites were identified by TLC, HPLC, GC-MS and other techniques.

ˇ We have studied the degradation of PE’s by immobilizing the bacterium in various matrices for its efficient utililization and reusability.

ˇ We have also isolated various enzymes involved in the metabolism of PE’s and partially purified by Gel-filtration, Ion-exchange chromatography and characterized the key enzymes involved in degradation.

ˇ We have studied the molecular aspects of degradation of PE’s by a Bacillus species and found that the genes of few enzymes involved in the degradation of PE’s are located on a plasmid in this bacterium.

ˇ We cured of the plasmid from the bacterium and characterized this plasmid-cured bacterium for its utilization of PE’s and other toxic chemicals.

In addition to my doctoral research, I was involved in a program (1999-2000) to study the antibiotic resistant properties of pathogenic microorganisms that are inducing resistant properties to most of the commonly used antibiotics. I have studied the mechanism of inactivation of certain antibiotics by the microorganisms particularly bacteria.

I have also standardized the protocol for developing an immuno-diagnostic kit for detection of fungal pathogen to most of the vegetable crops by raising antibodies against the intracellular protein of Fusarium sp. And studied the mechanism of toxin produced by this plant pathogen.

I was also engaged in screening of resistant and susceptible varieties of sunflower genotypes to A.helianthai (a fungal pathogen) and developing the RAPD markers for detection.

PUBLICATIONS

Javed Hussain Niazi, K., Theertha Prasad, D and Karegoudar, T.B. Initial degradation of Dimethylphthalate by Esterases from Bacillus species. FEMS Microbiol. Lett. 196 (2) 201-205, 2001.

Javed Hussain Niazi, K and Karegoudar, T.B. Degradation of Dimethylphthalate by Free and Immobilized cells of Bacillus sp. In Calcium alginate and Polyurethane foam. J. Environ. Sci. Health A36, No.6, 1134-1144, 2001.

MANUSCRIPTS SUBMITTED

Javed Hussain Niazi, K and Karegoudar, T.B. Degradation of Diethylphthalate by Free and Immobilized cells of Bacillus sp. In Calcium alginate and Polyurethane foam. 2001(Communicated)

Javed Hussain Niazi, K., Manohar, S and Karegoudar, T.B. Degradation of Dimethylphthalate by a bacterium. Arch. Microbiol. 2001 (Communicated)

MANUSCRIPTS UNDER PREPARATION

Javed Hussain Niazi, K and Karegoudar, T.B. Partial purification and characterization of esterases involved in degradation of Phthalate esters from Bacillus species.

PAPERS PRESENTED

Javed Hussain Niazi, K and Karegoudar, T.B. Degradation of Dimethylphthalate by a Bacillus species in the National Symposium on Environmental Issues- The 21st Century, Organized by National Environmental Science Academy, New Delhi during Dec, 12-15, 1998.

REFERENCES
1. Dr. T. B. Karegoudar
Reader in Biochemistry,
Gulbarga University,
Gulbarga-585 106
Off. Phone: 91 08472 445781
Res. Phone: 91 08472 445071
Email: goudartbk@rediffmail.com

2. Prof. V. H. Mulimani
Department of Biochemistry
Gulbarga University,
Gulbarga-585 106
Off Phone: 91 08472 445781
Res Phone: 91 08472 424481
Email: v_h_mulimani@rediffmail.com

3. Prof. G. R. Naik
Department of Biotechnology
Gulbarga University
Gulbarga-585 106
Off Phone: 91 08472 445781