Posted by on July 28, 19101 at 15:26:51:

ACADEMIC PROFILE
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Ph.D., Tissue Culture& Entomology, University of Madras,Chennai, India,1997-1999 Thesis highly commended awarded 2001-
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M.Phil., Botany-Tissue Culture, Bharathidasan University,Tiruchirappalli, India 1996-97 82 Distinction

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*M.Sc., Botany, M S University,Tinnelveli, India, 1993-1995 71

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*B.Sc., Botany, M K University,Madurai, India, 1988-1992 76

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* Won first prize for academic excellence

AWARDS & SCHOLARSHIPS
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* Senior Research Fellowship awarded by CSIR, New Delhi, India (1998-1999)

* Post Doctoral Fellowship awarded by Indian Institute of Science, Bangalore,
India (1999-2001)

* Winner of 2001 Student Travel Award from Society for In Vitro Biology, USA

RESEARCH EXPERIENCE
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1999 October'11th to till date working in the field of Plant Genetic Engineering under a project awarded by DBT through Indian Institute of Science,
which deals with the standardisation of efficient regeneration and
Agrobacterium mediated genetic transformation protocols for Solanum
melongena being carried out under the guidance of Prof. G. Lakshmi Sita.

Result: High frequency regeneration from all the explants and transformation
with GUS-INT has been standardised and confirmed by molecular analysis.
Presently work is being carried out with lepidopteran specific insect
resistance gene (Bt).
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1997-1999 in the field of Plant tissue culture and Transformation This project was awarded by CSIR as
Senior Research Fellowship and carried out under the guidance of Prof.
S. Ignacimuthu. This work dealt with creating Bruchid resistant pigeonpea
plants through Agrobacterium mediated genetic introduction of alpha amylase
inhibitor gene.
Result: Regeneration systems for three pulse crops namely Pisum sativum (pea), Vigna mungo
and Cajanus cajan has been standardised
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1996-1997(6 months)M.Phil project work on Multiple shoot induction and callus regeneration of
Cajanus cajan. at the biotechnology unit of St. Joseph's College,
Tiruchirappalli, India
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1995-1996 Worked as full time research scholar in tissue culture at the biotechnology unit of
St. Joseph's College, Tiruchirappalli, India
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1994-1995(6 months)M.Sc., project work on " The influence of commercial vitamins and growth
regulators on the regeneration of selected plants in vitro"
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Technical Expertise
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* Plant tissue culture

* Agrobacterium- mediated genetic transformation and molecular analysis transgenic
plants (Protein, DNA and RNA isolation, Cloning, PCR, Southern, Northern & Western
blots)

* Biochemical analysis of plants (estimation primary and secondary metabolites)

* Insect culture (pests like Helicoverpa, Callosobruchus etc) and their biological
performance at different conditions

List of Publications
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Ignacimuthu, S., Franklin, G., and Melchias, G., 1997. Multiple shoot formation and
in vitro fruiting of Vigna mungo L. Hepper, Curr. Sci., 73: 733-735

Franklin, G., Jeyachandran, R., Melchias, G., and Ignacimuthu, S., 1998. Multiple
shoot induction and regeneration of pigeon pea (Cajanus cajan L. Millsp) cv.
Vamban 1 from apical and axillary meristem. Curr. Sci., 74: 936-937.

Ignacimuthu, S., and Franklin, G., 1999. Regeneration of plantlets from cotyledon
and embryonal axes explants of Vigna mungo L. Hepper. Plant Cell, Tiss. Org. Cult.,
55:75-78.

Franklin, G., and Ignacimuthu, S., 1999. Regeneration of pea (Pisum sativum L.) from
seedling explants via organogenic nodule-like structures. Ind. J. Exp. Biol.,
37: 1119-1112.

Franklin, G., Jeyachandran, R., and Ignacimuthu, S., 1999. Factors affecting
regeneration of pigeonpea (Cajanus cajan L. Millsp) from mature embryonal axes.
Plant Growth Regulation. 30: 31-36

Franklin, G., and Ignacimuthu, S., 2000. Differential morphogenetic responses of
cotyledonary explants of Vigna mungo L. Hepper. Biologia Plantarum. 43: 157-160.

Franklin, G., and Ignacimuthu, S., 2000. Precocious in vitro flowering and fruiting
of multiple shoots of green pea (Pisum sativum L.). Euphytica . 115: 65-73.

Franklin, G., and Lakshmi Sita, G., 2001. Genetic transformation in two important
vegetable crops: Hot chillies (Capsicum annuum ) and eggplant (Solanum melongena L.),
In: Plant genetic engineering Vol :3, Improvement of commercial plants;
Eds Rana P Singh and Pawan K Jaiwal, New Delhi (in press).

Franklin, G., and Lakshmi Sita, G., 2001. High efficiency transformation of eggplant
(Solanum melongena L.) by Agrobacterium tumefaciens. Transgenic Res., (communicated)

Papers Presented in Conferences
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Franklin, G., and Ignacimuthu S., 1997 (November 20-22). Callus induction and shoot
organogenesis in Cajanus cajan L. Millsp. National Conference on impact of
Biotechnology and Modern Horticulture on Rural Development, Jadavpur University,
Calcutta, India (Oral).

Franklin, G., Jeyachandran, R., and Ignacimuthu, S., 1999 (July). Factors affecting
regeneration of pigeonpea (Cajanus cajan L. Millsp) from mature embryonal axes.
XVI International Botanical Congress, St. Louis, USA. (Poster)

Franklin, G., and Lakshmi Sita, G., 2001 (June 16-20). High efficiency transformation
of eggplant (Solanum melongena L.) by Agrobacterium tumefaciens. 2001 Congress on
In Vitro Biology, St. Louis, USA. (Oral)

REFERENCES
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Prof. G. Lakshmi Sita,
Dept of Microbiology & Cell Biology,
Indian Institute of Science,
Bangalore 560 012, INDIA,
Phone: 080-3092414
e-mail: sitagl@mcbl.iisc.ernet.in
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Rev. Prof. S. Ignacimuthu s.j,
Vice Chancellor, Bharathiar University, Coimbatore,
Tamil Nadu, INDIA,
Phone: 0422-422222,
Cell: 9843079010
e-mail: imuthu@hotmail.com
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Dr. R.V Sairam, Research Manager,
Plant Science Research Center
4223, Wolfe Hall, The university of Toledo,
Ohio 43606, USA
Phone: 419-530 1587,
Fax : 419-530 1539
Cell : 419-787 4976,
email: SRudrab@UTNet.UToledo.Edu
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Declaration

All the above mentioned details are true to the best of my knowledge and belief