Posted by on September 23, 19102 at 20:02:36:

CURRICULUM VITAE
Of
Vijaya sekhar Venati
Adress:S/O V.Dhanunjaya Reddy
Mavillapadu, Doravarisatram (Mandal)
Nellore-524123,Andhra pradesh,INDIA
MOBILE:9849046676 E-mail: vijes2000@yahoo.com

Academic Qualifications
·2000,Master of science (Applied Microbiology) from Universuty of Madras.
·1998,Bachelor of Science (Botany, Zoology, Chemistry) from Sri Venkateswara University.
·2001,Diploma in Bioinformatics.
Project:
Isolation of microorganisms associated with bovine
Mastitis:
Description:Inflammation of the mammary gland caused by Microorganisms, usually bacteria that invade the udder, multiply and produce toxins that are harmful to the mammary gland.
Mastitis occurs when the udder becomes inflamed because leukocytes are released into the mammary gland in response to invasion of the teat canal, usually by bacteria. These bacteria multiply and produce toxins that cause injury to milk secreting tissue and various ducts throughout the Mammary gland. Elevated leukocytes, or somatic cells, cause a reduction in milk production and alter milk composition. These changes in turn adversely affect quality and quantity of dairy products.
Mastitis Causing Bacteria: The most common mastitis pathogens are found either in the udder (contagious pathogens) or the cow’s surroundings (environmental pathogens), such as bedding, manure, soil, etc. Contagious mastitis pathogens (Staphylococcus aureus, Streptococcus agalactiae) are spread from infected udders to “clean” udders during the milking process through contaminated Teatcup liners, milkers’ hands, paper or cloth towels used to wash or dry more than one cow, and possibly by flies. Although new infections by environmental pathogens (other streptococci such as Str. uberis and Str.dysgalactiae and coliforms such as Escherichia coli and Klebsiella) can occur during milking, primary exposure appears to be between milkings. Coliform infections are usually associated with unsanitary environment.
Mastitis reduces milk yield and alters milk composition. The magnitude of these changes in individual cows varies with the severity and duration of the infection and the causative microorganisms.
Mastitis is almost always caused by bacteria. These microorganisms produce toxins that can directly damage milk-producing tissue of the mammary gland, and the presence of bacteria initiates inflammation within the mammary tissue in an attempt to eliminate the invading microorganisms. The inflammation contributes to decreased milk production and is primarily responsible for the compositional changes observed in milk from infected quarters and cows. In general, compositional changes involve an increase in blood components present in milk and a decrease in normal milk constituents.

Conclusion about project:
50 cases of bovine Mastitis infected milk samples studied, the causative agents were the staphylococcus aureus, pseudomonas, Escherichia coli and Klebsiella pneumoniae.The most predominant gram positive organism was staphylococcus aureus(50%),the most predominant gram negative organisms were pseudomonas (30%), Escherichia coli(14%) and Klebsiella pneumoniae(6%).
These organisms showing resistance to Ampicillin, Penicillin-G, Cephotaxime, Co-trimoxazole and were sensitive to newer antibiotics such as Ciproflaxacin, Danoflaxacin, Gentamicin, Tetracycline followed by Chloramphenicol.

Project 2: Total genomic characterization of staphylococcus aureus by RFLP.

Staphylococcus aureus is a major nosocomial pathogen that causes a range of diseases, including endocarditis, osteomyelitis, pneumonia, toxic-shock syndrome, food poisoning, carbuncles, and boils. In the early 1950s, acquisition and spread of beta-lactamase-producing plasmids thwarted the effectiveness of penicillin for treating S. aureus infections. Methicillin resistant Staphylococcus aureus (MRSA) is an organism that is frequently transmitted in Hospitals and perinatal units.At present several molecular typing systems are in use for monitoring the outbreaks of infections caused by staphylococcus aureus, particularly RFLP

In This study the parallel use of DNA restriction endonuclease to digest the total genome was standardized to look into the difference among standard strain (ATCC), Lab adopted strain and wild clinical isolates of methicillin resistant strains. Whole DNA isolated from cell of each strain and digested with Hind III showed different band patterns.

Molecular Biology Techniques known:
1. Isolation and culturing of various Micro-organisms
2. Staining Methods
3. DNA isolation
4. Restriction mapping &RFLP
5. Gel electrophoresis
6. Northern blotting
7. Southern blotting
8. Western blotting
9. PCR
10.Thin layer chromatography (TLC)

Bioinformatics skill
Genomics
Analysis of Bacterial, Eukaryotic genome databases.
Analysis of Mutations, SNP’s, Polymorphism.
Gene expression Analysis
Proteomics
Analysis of Protein databases
Methods for 2ry Protein structure prediction
Homology modelling and Hidden morkov modeling
Packages / Tools
EMBOSS, Vector-NTI, FASTA, BLAST, Rasmol, Phylodraw, ClustalX,J-Pred ,Procheck ,HMMER2 Modeller ,Pattern search,genie,Promotor prediction, Splice site prediction etc

Computer skills:
Unix/MS-Dos
Windows
MS-Office, Excel, Power point, Front page.
C,C++
PL/SQL, SQL
PERL

Work Experience:

12months of experience in the field of bioinformatics as bioinformatician
Organization: Genhelic bioinformatics Pvt.Ltd
Road no 7, Banjara Hills, Hyderabad.
Present job :working as Microbiologist in Manjeera mineral water (P)Ltd ,since may 9th 2002.
Fasalwadi village,sangareddy,Medak (dist)

Personal Profile
Fathers Name : Dhanunjaya Reddy .v
Date Of Birth : 1st January 1978
Strengths : Hard Working, Quick Grasping.

Nationality: Indian
Languages Known : English,Telugu, Hindi & Tamil.
Permanenat Address: S/O V.Dhanunjaya Reddy
Mavillapadu,
Doravarisatram (Mandal)
Nellore-524123
Andhra pradesh,INDIA