Microbiologist

Microbiologist


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Posted by on August 26, 19103 at 22:41:48:


Mr. Preetinder pal Singh
House. No. 92
V.P.O. Narangwal Kalan
Teh/Distt. Ludhiana -Punjab India 141203 Phone 0161-2863792
E-mail: nony11@rediffmail.com

Preetinder Pal Singh
_______________________________________________________________________

OBJECTIVE : Seeking a professional position in the field of Biotechnology.

Qualifications:

M.Sc. Biotechnology
Punjabi University Patiala, Punjab
With first class (68.75%), 2003


B.Sc. Industrial Microbiology
Govt. College, Ludhiana
(Punjab University, Chandigarh)
With first class (66.5%), 2001



Specialized Courses: Courses which include a lab
Microbiology Immunology Molecular Biology and Genetics Biochemistry
Biochemical Engineering Fermentation and Food Technology Enzymology and Enzyme Technology Genetic Engineering
Environmental And Commercial Biotechnology Tissue and Cell Culture Technology Industrial Microbiology Microbial Genetics

Technical skills:
Gene manipulation techniques: Isolation and purification of plant, bacterial
genomic and plasmid DNA, restriction enzyme digestion, ligation, transformation,
agarose gel-electrophoresis, PCR based amplification of DNA fragments, silver
staining method.
Microbiological Techniques: Pure culture methods, microbial quality control of
water, maintainance and reactivation of cultures
Fermentation techniques: Fermentative production of organic acids and ethanol using free-immobilized cells.
Production of alcoholic beverages--beer, wine and vinegar using free-immobilized cells.
Other techniques: Lowry Protein Assay, protein analysis by SDS-PAGE, separation analysis (HPLC, thin-layer chromatography, ion-exchange chromatography, gel filtration, spectrophotometry).

PROFESSIONAL EXPERIENCE:
06/06/01 to 21/07/01

Central Institute of Medicinal And Aromatic Plants, Lucknow (CSIR Lab)
Joining CIMAP’s summer school to learn the basic techniques in molecular biology, microbiology and plant tissue culture.
Working project: Isolation of multiple drug resistant mutants by NTG induced mutagenesis in case of Mycobacterium smegmatis Mc(2)155 - a study on drug resistance in Tuberculosis.
13/05/00 to 13/06/00

Kandhari Beverages Ltd (The Coca- Cola).
Katha- Baddi, Teh. Nalagarh, Distt. Solan (H.P)
On Job Training (OJT) for one month in Microbial Quality Control Section.
Learning basic techniques of microbiological testing of water and syrup/beverages.
(Swab Sampling, Membrane filter technique, MPN, Total Count using M-TGE Media, Feacal coliform count using M-F Endo broth, water purification systems).
05/05/1999 to 20/06/1999

Verka Milk Plant, Ludhiana, Punjab.
On Job Training (OJT) for one month in Microbial Quality Control Section.
Learning basic techniques of microbiological testing of dairy and dairy products,
(Pasteurization, EMB Test, detection of urea, metals and other impurities in milk products.)

Research work:
Project title: “Media optimization and fermentative production of pediocin using different strains of Pediococcus acidilactici.”
The study was undertaken to assess the feasibility of pediocin production in an economically cheaper media as compared to commercially available MRS medium. Media optimization was done using cheap substrates; molasses and corn steep liquor along with other components of MRS to obtain the maximum production at minimum cost viz. a viz. three different strains of Pediococcus acidilactici. From the results it was also inferred that the pediocin, an antimicrobial peptide has bactericidal or bacteriostatic effect on other (usually closely related) species, some of which are pathogenic including E. faecalis and Listeria monocytogens.

Personal details:

Date of Birth Feb 05, 1981 (
Father’s name S. Surjeet Singh
Mother’s name Mrs. Balwant Kaur
Nationality Indian
Sex Male (Single)


REFERENCES:

Dr. (Mrs.) P. P. Balgir
Reader,
Department of Biotechnology
Punjabi University,
Patiala, Punjab
INDIA-147002

Dr. Aruna Bhatia
Head,
Department of Biotechnology
Punjabi University,
Patiala, Punjab
INDIA-147002




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